Due to its hydrophobic nature 25OH Vitamin D, and other Vitamin D metabolites, circulate on binding proteins. About 90% of the total circulating 25OH Vitamin D is bound to the so-called Vitamin D Binding Protein (VDBP or DBP). The remaining 10% are bound to albumin, the main protein of human blood plasma. Although the affinity of albumin towards 25OH Vitamin D is much lower than the affinity of VDBP, the high concentration of albumin compensates for this difference.
BiosPacific offer a number of Vitamin D antibodies and antigens that will allow you to custom tailor your assay. 25OH Vitamin D has to be released from its binding proteins in any 25OH Vitamin D assay. BiosPacific supply the required Vitamin D release solutions to perform this release. Please get in touch if you would like to discuss any of these reagents